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InVivoMAb Anti-Human OPN/SPP1 (Iv0103)
Overview
Catalog No. HY207010
Host species Human
Species reactivity Human
Form Liquid
Storage buffer 0.01M PBS, pH 7.4.
Purity >95% as determined by SDS-PAGE.
Clonality Monoclonal
Isotype IgG1, kappa
Applications ELISA, FCM, Neutralization
Target Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, SPP-1, Urinary stone protein, Uropontin, SPP1, BNSP, OPN, PSEC0156
Purification Protein A/G purified from cell culture supernatant.
Endotoxin level Please contact with the lab for this information.
Accession P10451
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone IDIv0103
Note For research use only. Not suitable for clinical or therapeutic use.
Images


Flow Cytometry
Flow-cytometry using anti-human OPN antibody.HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human OPN antibody monoclonal antibody (Catalog # HY207010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

SDS-PAGE
SDS-PAGE for InVivoMAb Anti-Human OPN/SPP1 (Iv0103).

Western blot
Western blot analysis was performed using anti-OPN/SPP1 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human OPN/SPP1 (Catalog No: HY207012)
Lane 2: negative control