InVivoMAb Anti-Human CSF1/M-CSF (Iv0052)

Catalog No.	HY515010
Host species	Human
Species reactivity	Human
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4.
Purity	>95% as determined by SDS-PAGE.
Clonality	Monoclonal
Isotype	IgG1, kappa
Applications	ELISA, FCM, Neutralization
Target	CSF1, Macrophage colony-stimulating factor 1, CSF-1, Lanimostim, M-CSF, MCSF
Purification	Protein A/G purified from cell culture supernatant.
Endotoxin level	Please contact with the lab for this information.
Accession	P09603
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID	Iv0052
Note	For research use only. Not suitable for clinical or therapeutic use.

Bioactivity	SEC-HPLC detection for InVivoMAb Anti-Human CSF1/M-CSF (ABV0114).
Flow Cytometry	Flow-cytometry using anti-human CSF1 antibody.MG-63 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CSF1 antibody monoclonal antibody (Catalog # HY515010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE	SDS-PAGE for InVivoMAb Anti-Human CSF1/M-CSF (Iv0052).
Flow CytoMetry	Flow-cytometry using FITC anti-human CSF1 antibody. MG-63 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CSF1 monoclonal antibody (Catalog: HY515010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry	Flow-cytometry using PE anti-human CSF1 antibody. MG-63 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CSF1 monoclonal antibody (Catalog: HY515010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry	Flow-cytometry using APC anti-human CSF1 antibody. MG-63 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CSF1 monoclonal antibody (Catalog: HY515010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.