Lambda Protein Phosphatase (Lambda PP) is a Mn2+-dependent protein phosphatase with activity towards phosphorylated serine, threonine and tyrosine residues. One unit is defined as the amount of enzyme that hydrolyzes 1 nmol of p-Nitrophenyl Phosphate (50 mM) in 1 minute at 30°C in a total reaction volume of 50 µl.
Lambda Protein Phosphatase (Lambda PP)
Overview
Catalog No.
VK401012
Form
Liquid
Storage buffer
100 mM NaCl, 50 mM HEPES, pH 7.5, 0.1 mM MnCl2, 0.1 mM EDTA, 2 mM DTT, 0.01% Brij35, and 50% glycerol.
Purity
>90% as determined by SDS-PAGE.
Applications
Remove phosphate groups from phosphorylated serine, threonine and tyrosine residues in proteins.
Endotoxin level
Please contact with the lab for this information.
Expression system
E. coli
Protein length
Lambda PP is cloned from Escherichia phage lambda and expressed in E.coli.
Nature
Recombinant
Predicted molecular weight
27.32 kDa
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.
Species
Escherichia phage lambda (Bacteriophage lambda)
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Note
Avoid repeated freeze/thaw cycles. For research use only.
Description
Images
SDS-PAGE
SDS-PAGE for Lambda Protein Phosphatase (Lambda PP)