Research Grade Alemtuzumab
Overview
Storage buffer
0.01M PBS, pH 7.4.
Purity
>95% purity as determined by SDS-PAGE.
Applications
ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target
Human epididymis-specific protein 5, CDW52, CAMPATH-1 antigen, Cambridge pathology 1 antigen, He5, HE5, Epididymal secretory protein E5, CD52, CDw52
Purification
Protein A/G purified from cell culture supernatant.
Endotoxin level
Please contact the lab for this information.
Expression system
Mammalian Cells
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C.
Alternative name
Campath-1H, LDP-03, 216503-57-0
Note
For research use only. Not suitable for clinical or therapeutic use.
Images
Bioactivity
SEC-HPLC detection for Research Grade Alemtuzumab.
SDS-PAGE
SDS-PAGE for Research Grade Alemtuzumab.
Flow Cytometry
Flow-cytometry using anti-human CD52 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD52 monoclonal antibody (Catalog HB894016) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.