Research Grade Abatacept
Overview
Catalog No.
HW630296
Host species
Human
Species reactivity
Human
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4.
Purity
>95% purity as determined by SDS-PAGE.
Clonality
Monoclonal
Isotype
IgG1-Fc
Applications
ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Target
CD80, BB1, T-lymphocyte activation antigen CD80, CD28LG, B7, CD28LG1, LAB7, Activation B7-1 antigen, CTLA-4 counter-receptor B7.1, BU63, B70, CD28LG2, CTLA-4 counter-receptor B7.2, Activation B7-2 antigen, T-lymphocyte activation antigen CD86, CD86, FUN-1
Purification
Protein A/G purified from cell culture supernatant.
Endotoxin level
Please contact the lab for this information.
Expression system
Mammalian Cells
Accession
P33681 &/or P42081
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C for short-term storage (1-2 weeks). Store at -20°C for up to 12 months. For long-term storage, store at -80°C.
Note
For research use only. Not suitable for clinical or therapeutic use.
Images
Bioactivity
SEC-HPLC detection for Research Grade Abatacept.
Flow CytoMetry
Flow-cytometry using APC anti-human CD80 &/or CD86 antibody. Raji cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD80 &/or CD86 monoclonal antibody (Catalog: HW630296, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry
Flow-cytometry using PE anti-human CD80 &/or CD86 antibody. Raji cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD80 &/or CD86 monoclonal antibody (Catalog: HW630296, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.