Anti-MLST8 Polyclonal Antibody
Overview
Catalog No. |
HV195014 |
Host species |
Rabbit |
Species reactivity |
Human, Mouse, Bos taurus, Danio rerio, Rat, Xenopus laevis, Xenopus tropicalis |
Immunogen |
E. coli - derived recombinant Human MLST8 (Tyr68-Trp247). |
Form |
Liquid |
Storage buffer |
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. |
Clonality |
Polyclonal |
Isotype |
IgG |
Applications |
ELISA, IHC, WB |
Target |
GBL, TORC subunit LST8, Mammalian lethal with SEC13 protein 8, G protein beta subunit-like, Gable, Protein GbetaL, mLST8, LST8, MLST8, Target of rapamycin complex subunit LST8 |
Purification |
Purified by antigen affinity column. |
Accession |
Q9BVC4 |
Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
Product Usage Information |
Application | Dilution |
---|
ELISA | 1:5000-1:20000 | IHC | 1:50-1:500 | WB | 1:500-1:2000 |
|
Note |
For research use only. |
Images
 Immunofluorescence |
HepG2 cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting MLST8 (HV195014) at a concentration of 10.8 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of MLST8 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue). |